Nicolas F. Villarino

Nicolas F. Villarino,

Vet. Med., D.V.Sc, Ph.D.

Associate Professor
Department of Veterinary Clinical Sciences
Director Pharmacokinetics Laboratory
Diplomate, American College of Veterinary Clinical Pharmacology

Office: ABDF
Phone: 509-335-9831


Postdoctoral Research Associate at the University of Tennessee (2012-2014) (Immunology)
Ph.D. Veterinary Pharmacology at the University of Tennessee (2012) (Pharmacology)
Residency in Veterinary Clinical Pharmacology. The University of Tennessee (2007-2011)
Internship in Equine Medicine and Surgery. California (2006-2007)
Doctor in Veterinary Sciences, Veterinary Pharmacology, Universidad Nacional de La Plata, Argentina (2006) (Pharmacology).
Veterinary Medicine, Universidad Nacional de La Plata, Argentina (2000)

Nicolas Villarino

Research Interests

To individualize pharmacological treatments for animals by integrating pharmacokinetics, pharmacodynamics, pharmacogenomics and metabolomics.


  • Villarino, NF., Gary R. LeCleir, Joshua E. Denny, Sarah S. Sloan, Steven W. Wilhelm, and Nathan W. Schmidt. Composition of the gut microbiota modulates the severity of malaria. Proc Natl Acad Sci U S A. 2016 23;113(8):2235-40.

  • Rivera Vélez SM., Morassi A., Court MH., Slovak JE, Villarino NF*. Development and validation of an ultrafast chromatographic method for quantification of the immunosuppressant mycophenolic acid in canine, feline and human plasma. J, Pharm. Biomedical Analysis. 2016 Volume 131, 94-102.

  • White C., Villarino NF., Gunasov V. and Schmidt NW. Plasmodium suppresses expansion of T cell responses to heterologous infections. J Immunol. 2015 Jan 15;194(2):697-708.

  • Villarino N., Denny, JE., and Schmidt NW. Antimalarial Activity of Tulathromycin in a Murine Model of Malaria. Antimicrob Agents Chemother. 2015;59(6):3672-4.

  • Sanz MG., Villarino N., Horohov DW., VapA-specific IgG and IgG subclasses responses after natural infection and experimental challenge of foals with Rhodococcus equi. Vet Immunol Immunopathol. 2015 Mar 15;164(1-2):10-5.

  • Rubio-Martínez LM, Bracamonte JL, Tompkins S, Villarino NF. Transthecal Arthroscopy of the Palmar Distal Interphalangeal Joint in the Horse: A Cadaver Study. Vet Surg. 2015 Nov;44(8):939-43

  • Mahne H., L. Rubio-Martinez L., Marais H., Villarino N., and Rioja E.. Clinical and pharmacokinetic effects of regional or general anaesthesia on intravenous regional limb perfusion with amikacin in horses. Equine Vet J. 2014 May;46(3):375-9.

  • Villarino N., Brown SA, Martín-Jiménez T. Pharmacokinetics of Tulathromycin: a pulmonary perspective. J Vet Pharmacol Ther. 2014 Jun;37(3):211-21.

  • Villarino N. and Schmidt, NW. CD8+ T Cell Responses to Plasmodium and Intracellular Parasites. Current Immunology Reviews, 9, (3), August 2013 169-178.

  • Cox S., Villarino N., et al., Disposition of Firocoxib in Equine Plasma After An Oral Loading Dose and a Multiple Dose Regimen. Vet J. 2013 Nov;198(2):382-5.

  • Villarino N., Brown SA, Martín-Jiménez T. The role of the macrolide tulathromycin in veterinary medicine. The Veterinary Journal. 2013 Nov;198(2):352-7.

  • Villarino N., Garcia-Tapia D, Lesman S, Lucas M, Robinson J, Brown SA, Martin-Jimenez T. An acute reversible experimental model of pneumonia in pigs: time-related histological and clinical signs changes. J Vet Pharmacol Ther. 2013 Aug;36(3):341-7.

  • Villarino N., Lesman S, Fielder A, García-Tapia D, Cox S, Lucas M, Robinson J, Brown SA, Martín-Jiménez T. Pulmonary pharmacokinetics of tulathromycin in swine. Part I: Lung homogenate in healthy pigs and pigs challenged intratracheally with lipopolysaccharide of Escherichia coli. J Vet Pharmacol Ther. 2013 Aug;36(4):329-39.

  • Villarino N., Lesman S, Fielder A, García-Tapia D, Cox S, Lucas M, Robinson J, Brown SA, Martín-Jiménez T. Pulmonary pharmacokinetics of tulathromycin in swine. Part 2: Intra-airways compartments. J Vet Pharmacol Ther. 2013 Aug;36(4):340-9.

  • Villarino N., Martín-Jiménez T. Pharmacokinetics of macrolides in foals. J Vet Pharmacol Ther. 2013 Aug;36(3):1-13.

  • Villarino N., Brown SA, Martín-Jiménez T. Pharmacokinetics of tulathromycin in healthy and neutropenic mice challenged intranasally with lipopolysaccharide from Escherichia coli. Antimicrob Agents Chemother. 2012 Aug;56(8):4078-86.

  • Sanchez Teran A., Rubio Martinez L., Villarino N., Sanz, M. Effects of repeated intra-articular administration of amikacin on serum amyloid A, total protein and nucleated cell count in synovial fluid from healthy horses. Equine Vet J. 2012 Dec;44 Suppl 43:12-6.

  • Cox S, Villarino N., Doherty T. Determination of oral tramadol pharmacokinetics in horses. Res Vet Sci. 2010 Oct;89(2):236-41.

  • Villarino N., Cox S., Yarbrough J., Martín-Jiménez T. Determination of carboplatin in canine plasma by high-performance liquid chromatography. Biomed Chromatogr. 2010 Aug;24(8):908-13.

  • Ferrante M., Villarino N., Andreata A. & Landoni MF. Impacto de la Remoción del Estrato Córneo sobre la Penetración de Diclofenac a Través de Piel Equina Lat. Am. J. Pharm. 2008.27 (2): 185-190 2008.

  • Villarino N., Vispo T., Ferrante M. and Landoni MF. 2006 Inefficacy of Topical Diclofenac in Arthritic Horses. American Journal of Animal and Veterinary Sciences 1 (1): 8-12.

  • Villarino N. and Landoni MF. 2006 Transdermal Drug Adminstration: a therapeutical alternative. The Veterinary Analect. Vol 26 (1).

  • Verde CR.; Villarino N., Simpson MI., Frigoli A., Landoni MF. (2006) Study of therapeutic and secondary effects of native and low molecular weight chondroitin sulfate after 10 weeks of oral administration in equines with experimentally induced chronic arthritis. Redvet. 2006;7;(1):1-23.

  • Villarino N. and Landoni MF. 2006. Transdermal drug delivery: a new frontier in the administration of therapeutic drugs to veterinary species. The Veterinary Journal 2006. 172, 2. 200-201.