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PrIMe research team develops assay for immunosuppressant drug to improve its safe use in dogs and cats

September 1, 2016
Mycophenolic acid (MPA) is the active metabolite of the immunosuppressant prodrug mycophenolate mofetil. In this study, we developed and validated a novel ultra-high performance liquid chromatography (UHPLC) method for the rapid quantification of MPA in plasma from dogs, cats and humans. The advantages of this method include: high sensitivity and reproducibility over a wide range of MPA plasma concentrations, small sample volume and easy sample preparation. By combining isocratic conditions with a UHPLC column containing solid core particles, we were able to elute MPA within 3.0 min. The very short chromatographic analysis time makes this method ideal to study the disposition of MPA in large batches of plasma samples and/or monitor plasma drug concentrations, as recommended for patients that require optimized immunosuppression.
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